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Miltenyi Biotec mouse anti human monoclonal antibodies
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Cedarlane rat anti human mac2
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Cedarlane anti mac2
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Cedarlane mac2
( A ) Schematic outlining experimental design and the generation of the SMC transgenic ADAMTS7 mouse. Created with BioRender.com. ( B ) ORO staining of the en face aorta and quantification of lesion area. n = 9. ( C ) Representative images of H&E-stained aortic root sections, quantification of plaque areas ( n = 6). Scale bar: 500 μm. ( D ) Representative images of H&E-stained aortic root sections with necrotic core outlined in dotted line ( n = 6 mice). Scale bar: 300 μm. ( E ) Representative images of aortic root sections stained against α-SMA (Cy3; red), <t>MAC2</t> (Alexa Fluor 488 green), and cell nuclei (DAPI blue) with their subsequent quantification relative to lesion area ( n = 6). Scale bar: 300 μm. ( F ) Representative Picrosirius red staining of aortic root lesions with bars indicating fibrous cap thickness and quantification of fibrous cap thickness ( n = 6). Scale bar: 300 μm. ( G ) Schematic outlining experimental design and the generation of the EC transgenic ADAMTS7 mouse. Created with BioRender.com. ( H ) ORO staining of the en face aorta and quantification of lesion area. n = 3–4. ( I ) Representative images of H&E-stained aortic root sections and quantification of plaque areas ( n = 5–6). Scale bar: 500 μm. **** P < 0.0001, * P < 0.05. Statistics were analyzed using a 2-tailed Student’s t test.
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Cedarlane mouse
( A ) Schematic outlining experimental design and the generation of the SMC transgenic ADAMTS7 mouse. Created with BioRender.com. ( B ) ORO staining of the en face aorta and quantification of lesion area. n = 9. ( C ) Representative images of H&E-stained aortic root sections, quantification of plaque areas ( n = 6). Scale bar: 500 μm. ( D ) Representative images of H&E-stained aortic root sections with necrotic core outlined in dotted line ( n = 6 mice). Scale bar: 300 μm. ( E ) Representative images of aortic root sections stained against α-SMA (Cy3; red), <t>MAC2</t> (Alexa Fluor 488 green), and cell nuclei (DAPI blue) with their subsequent quantification relative to lesion area ( n = 6). Scale bar: 300 μm. ( F ) Representative Picrosirius red staining of aortic root lesions with bars indicating fibrous cap thickness and quantification of fibrous cap thickness ( n = 6). Scale bar: 300 μm. ( G ) Schematic outlining experimental design and the generation of the EC transgenic ADAMTS7 mouse. Created with BioRender.com. ( H ) ORO staining of the en face aorta and quantification of lesion area. n = 3–4. ( I ) Representative images of H&E-stained aortic root sections and quantification of plaque areas ( n = 5–6). Scale bar: 500 μm. **** P < 0.0001, * P < 0.05. Statistics were analyzed using a 2-tailed Student’s t test.
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Cedarlane rat anti mouse mac 2
( A ) Schematic outlining experimental design and the generation of the SMC transgenic ADAMTS7 mouse. Created with BioRender.com. ( B ) ORO staining of the en face aorta and quantification of lesion area. n = 9. ( C ) Representative images of H&E-stained aortic root sections, quantification of plaque areas ( n = 6). Scale bar: 500 μm. ( D ) Representative images of H&E-stained aortic root sections with necrotic core outlined in dotted line ( n = 6 mice). Scale bar: 300 μm. ( E ) Representative images of aortic root sections stained against α-SMA (Cy3; red), <t>MAC2</t> (Alexa Fluor 488 green), and cell nuclei (DAPI blue) with their subsequent quantification relative to lesion area ( n = 6). Scale bar: 300 μm. ( F ) Representative Picrosirius red staining of aortic root lesions with bars indicating fibrous cap thickness and quantification of fibrous cap thickness ( n = 6). Scale bar: 300 μm. ( G ) Schematic outlining experimental design and the generation of the EC transgenic ADAMTS7 mouse. Created with BioRender.com. ( H ) ORO staining of the en face aorta and quantification of lesion area. n = 3–4. ( I ) Representative images of H&E-stained aortic root sections and quantification of plaque areas ( n = 5–6). Scale bar: 500 μm. **** P < 0.0001, * P < 0.05. Statistics were analyzed using a 2-tailed Student’s t test.
Rat Anti Mouse Mac 2, supplied by Cedarlane, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bio X Cell mouse
( A ) Schematic outlining experimental design and the generation of the SMC transgenic ADAMTS7 mouse. Created with BioRender.com. ( B ) ORO staining of the en face aorta and quantification of lesion area. n = 9. ( C ) Representative images of H&E-stained aortic root sections, quantification of plaque areas ( n = 6). Scale bar: 500 μm. ( D ) Representative images of H&E-stained aortic root sections with necrotic core outlined in dotted line ( n = 6 mice). Scale bar: 300 μm. ( E ) Representative images of aortic root sections stained against α-SMA (Cy3; red), <t>MAC2</t> (Alexa Fluor 488 green), and cell nuclei (DAPI blue) with their subsequent quantification relative to lesion area ( n = 6). Scale bar: 300 μm. ( F ) Representative Picrosirius red staining of aortic root lesions with bars indicating fibrous cap thickness and quantification of fibrous cap thickness ( n = 6). Scale bar: 300 μm. ( G ) Schematic outlining experimental design and the generation of the EC transgenic ADAMTS7 mouse. Created with BioRender.com. ( H ) ORO staining of the en face aorta and quantification of lesion area. n = 3–4. ( I ) Representative images of H&E-stained aortic root sections and quantification of plaque areas ( n = 5–6). Scale bar: 500 μm. **** P < 0.0001, * P < 0.05. Statistics were analyzed using a 2-tailed Student’s t test.
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Miltenyi Biotec biotec 130 112 969 20
( A ) Schematic outlining experimental design and the generation of the SMC transgenic ADAMTS7 mouse. Created with BioRender.com. ( B ) ORO staining of the en face aorta and quantification of lesion area. n = 9. ( C ) Representative images of H&E-stained aortic root sections, quantification of plaque areas ( n = 6). Scale bar: 500 μm. ( D ) Representative images of H&E-stained aortic root sections with necrotic core outlined in dotted line ( n = 6 mice). Scale bar: 300 μm. ( E ) Representative images of aortic root sections stained against α-SMA (Cy3; red), <t>MAC2</t> (Alexa Fluor 488 green), and cell nuclei (DAPI blue) with their subsequent quantification relative to lesion area ( n = 6). Scale bar: 300 μm. ( F ) Representative Picrosirius red staining of aortic root lesions with bars indicating fibrous cap thickness and quantification of fibrous cap thickness ( n = 6). Scale bar: 300 μm. ( G ) Schematic outlining experimental design and the generation of the EC transgenic ADAMTS7 mouse. Created with BioRender.com. ( H ) ORO staining of the en face aorta and quantification of lesion area. n = 3–4. ( I ) Representative images of H&E-stained aortic root sections and quantification of plaque areas ( n = 5–6). Scale bar: 500 μm. **** P < 0.0001, * P < 0.05. Statistics were analyzed using a 2-tailed Student’s t test.
Biotec 130 112 969 20, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Boster Bio antibody anti galectin 3
<t>Galectin-3</t> mRNA expression in chorionic villi of the SA and MA groups. The relative mRNA expression levels of galectin-3 were determined by RT-qPCR in chorionic villi tissue samples from patients in the SA and MA group. (A) The expression level of galectin-3 was significantly increased in the MA group compared with the SA group. (B) The expression of galectin-3 in early MA group (<4 weeks) is decreased compared with the SA group. The expression level of galectin-3 in the MA group gradually increases with time. *P<0.05 vs. SA group. SA, spontaneous abortion; MA, missed abortion; RT-qPCR, reverse-transcription quantitative polymerase chain reaction; 4 w, 4 weeks.
Antibody Anti Galectin 3, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fluidigm mac 2 m3 38 fluidigm eu153 3153026b ab 2814900
<t>Galectin-3</t> mRNA expression in chorionic villi of the SA and MA groups. The relative mRNA expression levels of galectin-3 were determined by RT-qPCR in chorionic villi tissue samples from patients in the SA and MA group. (A) The expression level of galectin-3 was significantly increased in the MA group compared with the SA group. (B) The expression of galectin-3 in early MA group (<4 weeks) is decreased compared with the SA group. The expression level of galectin-3 in the MA group gradually increases with time. *P<0.05 vs. SA group. SA, spontaneous abortion; MA, missed abortion; RT-qPCR, reverse-transcription quantitative polymerase chain reaction; 4 w, 4 weeks.
Mac 2 M3 38 Fluidigm Eu153 3153026b Ab 2814900, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Miltenyi Biotec apc
<t>Galectin-3</t> mRNA expression in chorionic villi of the SA and MA groups. The relative mRNA expression levels of galectin-3 were determined by RT-qPCR in chorionic villi tissue samples from patients in the SA and MA group. (A) The expression level of galectin-3 was significantly increased in the MA group compared with the SA group. (B) The expression of galectin-3 in early MA group (<4 weeks) is decreased compared with the SA group. The expression level of galectin-3 in the MA group gradually increases with time. *P<0.05 vs. SA group. SA, spontaneous abortion; MA, missed abortion; RT-qPCR, reverse-transcription quantitative polymerase chain reaction; 4 w, 4 weeks.
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Miltenyi Biotec human mouse
<t>Galectin-3</t> mRNA expression in chorionic villi of the SA and MA groups. The relative mRNA expression levels of galectin-3 were determined by RT-qPCR in chorionic villi tissue samples from patients in the SA and MA group. (A) The expression level of galectin-3 was significantly increased in the MA group compared with the SA group. (B) The expression of galectin-3 in early MA group (<4 weeks) is decreased compared with the SA group. The expression level of galectin-3 in the MA group gradually increases with time. *P<0.05 vs. SA group. SA, spontaneous abortion; MA, missed abortion; RT-qPCR, reverse-transcription quantitative polymerase chain reaction; 4 w, 4 weeks.
Human Mouse, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


( A ) Schematic outlining experimental design and the generation of the SMC transgenic ADAMTS7 mouse. Created with BioRender.com. ( B ) ORO staining of the en face aorta and quantification of lesion area. n = 9. ( C ) Representative images of H&E-stained aortic root sections, quantification of plaque areas ( n = 6). Scale bar: 500 μm. ( D ) Representative images of H&E-stained aortic root sections with necrotic core outlined in dotted line ( n = 6 mice). Scale bar: 300 μm. ( E ) Representative images of aortic root sections stained against α-SMA (Cy3; red), MAC2 (Alexa Fluor 488 green), and cell nuclei (DAPI blue) with their subsequent quantification relative to lesion area ( n = 6). Scale bar: 300 μm. ( F ) Representative Picrosirius red staining of aortic root lesions with bars indicating fibrous cap thickness and quantification of fibrous cap thickness ( n = 6). Scale bar: 300 μm. ( G ) Schematic outlining experimental design and the generation of the EC transgenic ADAMTS7 mouse. Created with BioRender.com. ( H ) ORO staining of the en face aorta and quantification of lesion area. n = 3–4. ( I ) Representative images of H&E-stained aortic root sections and quantification of plaque areas ( n = 5–6). Scale bar: 500 μm. **** P < 0.0001, * P < 0.05. Statistics were analyzed using a 2-tailed Student’s t test.

Journal: The Journal of Clinical Investigation

Article Title: ADAMTS7 promotes smooth muscle foam cell expansion in atherosclerosis

doi: 10.1172/JCI187451

Figure Lengend Snippet: ( A ) Schematic outlining experimental design and the generation of the SMC transgenic ADAMTS7 mouse. Created with BioRender.com. ( B ) ORO staining of the en face aorta and quantification of lesion area. n = 9. ( C ) Representative images of H&E-stained aortic root sections, quantification of plaque areas ( n = 6). Scale bar: 500 μm. ( D ) Representative images of H&E-stained aortic root sections with necrotic core outlined in dotted line ( n = 6 mice). Scale bar: 300 μm. ( E ) Representative images of aortic root sections stained against α-SMA (Cy3; red), MAC2 (Alexa Fluor 488 green), and cell nuclei (DAPI blue) with their subsequent quantification relative to lesion area ( n = 6). Scale bar: 300 μm. ( F ) Representative Picrosirius red staining of aortic root lesions with bars indicating fibrous cap thickness and quantification of fibrous cap thickness ( n = 6). Scale bar: 300 μm. ( G ) Schematic outlining experimental design and the generation of the EC transgenic ADAMTS7 mouse. Created with BioRender.com. ( H ) ORO staining of the en face aorta and quantification of lesion area. n = 3–4. ( I ) Representative images of H&E-stained aortic root sections and quantification of plaque areas ( n = 5–6). Scale bar: 500 μm. **** P < 0.0001, * P < 0.05. Statistics were analyzed using a 2-tailed Student’s t test.

Article Snippet: The sections then were stained using the following Abs and dilutions: 1:1,000 α-SMA-Cy3 (MilliporeSigma, C6198) and 1:500 MAC2 (Cedarlane, CL8942AP).

Techniques: Transgenic Assay, Staining

Galectin-3 mRNA expression in chorionic villi of the SA and MA groups. The relative mRNA expression levels of galectin-3 were determined by RT-qPCR in chorionic villi tissue samples from patients in the SA and MA group. (A) The expression level of galectin-3 was significantly increased in the MA group compared with the SA group. (B) The expression of galectin-3 in early MA group (<4 weeks) is decreased compared with the SA group. The expression level of galectin-3 in the MA group gradually increases with time. *P<0.05 vs. SA group. SA, spontaneous abortion; MA, missed abortion; RT-qPCR, reverse-transcription quantitative polymerase chain reaction; 4 w, 4 weeks.

Journal: Experimental and Therapeutic Medicine

Article Title: Expression of galectin-3 and apoptosis in placental villi from patients with missed abortion during early pregnancy

doi: 10.3892/etm.2019.7227

Figure Lengend Snippet: Galectin-3 mRNA expression in chorionic villi of the SA and MA groups. The relative mRNA expression levels of galectin-3 were determined by RT-qPCR in chorionic villi tissue samples from patients in the SA and MA group. (A) The expression level of galectin-3 was significantly increased in the MA group compared with the SA group. (B) The expression of galectin-3 in early MA group (<4 weeks) is decreased compared with the SA group. The expression level of galectin-3 in the MA group gradually increases with time. *P<0.05 vs. SA group. SA, spontaneous abortion; MA, missed abortion; RT-qPCR, reverse-transcription quantitative polymerase chain reaction; 4 w, 4 weeks.

Article Snippet: The samples were incubated with the primary antibody anti-galectin 3 (1:200; cat. no. PB9081; Boster Biotechnology, Wuhan, China) overnight at 4°C.

Techniques: Expressing, Quantitative RT-PCR, Real-time Polymerase Chain Reaction

Galectin-3 protein expression in chorionic villi of the SA and MA groups. The relative mRNA expression levels of galectin-3 were determined by ELISA in chorionic villi tissue samples from patients in the SA and MA group. (A) The galectin-3 protein expression level is significantly higher in the MA group compared with the SA group. (B) The galectin-3 protein expression level in the early MA group (<4 weeks) is not significantly different compared with the SA group; whilst the galectin-3 protein expression level in the late MA group (>4 weeks) was significantly higher compared with the SA group. *P<0.05 vs. SA group. SA, spontaneous abortion; MA, missed abortion; 4 w, 4 weeks.

Journal: Experimental and Therapeutic Medicine

Article Title: Expression of galectin-3 and apoptosis in placental villi from patients with missed abortion during early pregnancy

doi: 10.3892/etm.2019.7227

Figure Lengend Snippet: Galectin-3 protein expression in chorionic villi of the SA and MA groups. The relative mRNA expression levels of galectin-3 were determined by ELISA in chorionic villi tissue samples from patients in the SA and MA group. (A) The galectin-3 protein expression level is significantly higher in the MA group compared with the SA group. (B) The galectin-3 protein expression level in the early MA group (<4 weeks) is not significantly different compared with the SA group; whilst the galectin-3 protein expression level in the late MA group (>4 weeks) was significantly higher compared with the SA group. *P<0.05 vs. SA group. SA, spontaneous abortion; MA, missed abortion; 4 w, 4 weeks.

Article Snippet: The samples were incubated with the primary antibody anti-galectin 3 (1:200; cat. no. PB9081; Boster Biotechnology, Wuhan, China) overnight at 4°C.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay

Galectin-3 protein expression in SA and MA groups determined by IHC. Galectin-3 protein expression was examined in chorionic villi tissue samples from patients in the (A) negative control, (B) SA group and (C) MA group (magnification, ×400). Red arrows indicate galectin-3-positive expression in cells stained by DAB and blue arrows indicate cell nuclei counterstained with hematoxylin. SA, spontaneous abortion; MA, missed abortion; IHC, immunohistochemistry; DAB, diaminobenzidine.

Journal: Experimental and Therapeutic Medicine

Article Title: Expression of galectin-3 and apoptosis in placental villi from patients with missed abortion during early pregnancy

doi: 10.3892/etm.2019.7227

Figure Lengend Snippet: Galectin-3 protein expression in SA and MA groups determined by IHC. Galectin-3 protein expression was examined in chorionic villi tissue samples from patients in the (A) negative control, (B) SA group and (C) MA group (magnification, ×400). Red arrows indicate galectin-3-positive expression in cells stained by DAB and blue arrows indicate cell nuclei counterstained with hematoxylin. SA, spontaneous abortion; MA, missed abortion; IHC, immunohistochemistry; DAB, diaminobenzidine.

Article Snippet: The samples were incubated with the primary antibody anti-galectin 3 (1:200; cat. no. PB9081; Boster Biotechnology, Wuhan, China) overnight at 4°C.

Techniques: Expressing, Negative Control, Staining, Immunohistochemistry

Quantification of galectin-3 protein expression in SA and MA groups determined by IHC. SA, spontaneous abortion; MA, missed abortion; IHC, immunohistochemistry.

Journal: Experimental and Therapeutic Medicine

Article Title: Expression of galectin-3 and apoptosis in placental villi from patients with missed abortion during early pregnancy

doi: 10.3892/etm.2019.7227

Figure Lengend Snippet: Quantification of galectin-3 protein expression in SA and MA groups determined by IHC. SA, spontaneous abortion; MA, missed abortion; IHC, immunohistochemistry.

Article Snippet: The samples were incubated with the primary antibody anti-galectin 3 (1:200; cat. no. PB9081; Boster Biotechnology, Wuhan, China) overnight at 4°C.

Techniques: Expressing, Immunohistochemistry